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Targeted PCR for Vaginal Bacteria Detection Linked to Bacterial issue
Several novel bacterial species have been detected in subjects with bacterial vaginosis (BV) by using broad-range PCR assays, but this approach is insensitive for detecting minority species. We developed a series of taxon-directed 16S rRNA gene PCR assays for more sensitive detection of key vaginal bacteria. We sought to determine the prevalence of each species in the vagina, its association with BV, and the utility of PCR for the microbiological diagnosis of BV. Targeted PCR assays were developed for 17 vaginal bacterial species and applied to 264 vaginal-fluid samples from 81 subjects with and 183 subjects without BV. The results were compared to those of two widely accepted methods for diagnosing BV, the use of clinical findings (Amsel criteria) and the interpretation of vaginal-fluid Gram stains (Nugent criteria). Leptotrichia/Sneathia, Atopobium vaginae, an Eggerthella-like bacterium, Megasphaera species, and three novel bacteria in the order Clostridiales are among the bacterial species significantly associated with BV. PCR detection of either a Megasphaera species or one of the Clostridiales bacteria yielded a sensitivity of 99% and a specificity of 89% for diagnosis of BV compared to the Amsel clinical criteria and a sensitivity of 95.9% and a specificity of 93.7% compared to the Nugent criteria (Gram stain). PCR detection of one or more fastidious bacterial species is a more reliable indicator of BV than detection of bacteria, such as Gardnerella vaginalis, previously linked to BV, highlighting the potential of PCR for the diagnosis of BV.
Streamline scientific always aspire to build a transparent expert system to deliver the best in patient care and improve antibiotic stewardship. Our team is made of doctors and scientists who know how quality data and partnerships can amplify the effectiveness.
The experts at Streamline Scientific aim to build a system that can improve the laboratories sector and make it more efficient. We take charge of lab testing and bring crucial molecular insights closer to those who need it.
Our solutions are made to empower researchers and physicians with the best data, faster and more efficiently. We work and create partnerships with other immaculate groups to bring productive insights to you efficiently. Streamline Scientific works to optimise your lab and equip healthcare providers and researchers with optimum solutions and that too at a low cost. Want to know how?
Read our insightful blogs to get a deeper idea.Streamline scientific always aspire to build a transparent expert system to deliver the best in patient care and improve antibiotic stewardship. Our team is made of doctors and scientists who know how quality data and partnerships can amplify the effectiveness. The experts at Streamline Scientific aim to build a system that can improve the laboratories sector and make it more efficient. We take charge of lab testing and bring crucial molecular insights closer to those who need it.
Our solutions are made to empower researchers and physicians with the best data, faster and more efficiently. We work and create partnerships with other immaculate groups to bring productive insights to you efficiently. Streamline Scientific works to optimise your lab and equip healthcare providers and researchers with optimum solutions and that too at a low cost. Want to know how? Read our insightful blogs to get a deeper idea.
Alongside our national expansion, in 2022 Assurance rebranded as Streamline Scientific. Read more about how we got here.
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